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siha cervix squamous cancer cells cell lines  (ATCC)


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    ATCC siha cervix squamous cancer cells cell lines
    Siha Cervix Squamous Cancer Cells Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2611 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 2611 article reviews
    siha cervix squamous cancer cells cell lines - by Bioz Stars, 2026-02
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    Analytic sensitivity <t>of</t> <t>HPV-seq.</t> A, HPV-seq was conducted on fragmented <t>SiHa</t> genomic DNA at the indicated dilution. Hybrid capture baits targeted the indicated HPV-16 sequences. The lower limit of detection (LLOD) of HPV-seq was dependent on the use of dual-strand hybrid capture and the length of HPV-16 genome targeted by the baits. B, HPV-seq with full-length dual-strand hybrid capture (blue) provided an improvement in analytic sensitivity and LLOD (0.003%) as compared with hybrid capture for a single mutation (1%). C, Influence of multiple markers and sequencing depth on LLOD. Downsampling of HPV-seq data from full-length dual-strand hybrid capture demonstrates the dependence of the LLOD on the targeted length of HPV-16 genome (i.e., number of markers; right y -axis) and the sequencing depth ( x -axis). The probability of detecting the indicated number of HPV molecules (1, blue circles; 2, gray triangles; 5, yellow squares) is shown (left y -axis).
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    Analytic sensitivity <t>of</t> <t>HPV-seq.</t> A, HPV-seq was conducted on fragmented <t>SiHa</t> genomic DNA at the indicated dilution. Hybrid capture baits targeted the indicated HPV-16 sequences. The lower limit of detection (LLOD) of HPV-seq was dependent on the use of dual-strand hybrid capture and the length of HPV-16 genome targeted by the baits. B, HPV-seq with full-length dual-strand hybrid capture (blue) provided an improvement in analytic sensitivity and LLOD (0.003%) as compared with hybrid capture for a single mutation (1%). C, Influence of multiple markers and sequencing depth on LLOD. Downsampling of HPV-seq data from full-length dual-strand hybrid capture demonstrates the dependence of the LLOD on the targeted length of HPV-16 genome (i.e., number of markers; right y -axis) and the sequencing depth ( x -axis). The probability of detecting the indicated number of HPV molecules (1, blue circles; 2, gray triangles; 5, yellow squares) is shown (left y -axis).
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    ATCC cervix cancer cell line siha
    Analytic sensitivity <t>of</t> <t>HPV-seq.</t> A, HPV-seq was conducted on fragmented <t>SiHa</t> genomic DNA at the indicated dilution. Hybrid capture baits targeted the indicated HPV-16 sequences. The lower limit of detection (LLOD) of HPV-seq was dependent on the use of dual-strand hybrid capture and the length of HPV-16 genome targeted by the baits. B, HPV-seq with full-length dual-strand hybrid capture (blue) provided an improvement in analytic sensitivity and LLOD (0.003%) as compared with hybrid capture for a single mutation (1%). C, Influence of multiple markers and sequencing depth on LLOD. Downsampling of HPV-seq data from full-length dual-strand hybrid capture demonstrates the dependence of the LLOD on the targeted length of HPV-16 genome (i.e., number of markers; right y -axis) and the sequencing depth ( x -axis). The probability of detecting the indicated number of HPV molecules (1, blue circles; 2, gray triangles; 5, yellow squares) is shown (left y -axis).
    Cervix Cancer Cell Line Siha, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cervix cancer cell line siha/product/ATCC
    Average 99 stars, based on 1 article reviews
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    Analytic sensitivity of HPV-seq. A, HPV-seq was conducted on fragmented SiHa genomic DNA at the indicated dilution. Hybrid capture baits targeted the indicated HPV-16 sequences. The lower limit of detection (LLOD) of HPV-seq was dependent on the use of dual-strand hybrid capture and the length of HPV-16 genome targeted by the baits. B, HPV-seq with full-length dual-strand hybrid capture (blue) provided an improvement in analytic sensitivity and LLOD (0.003%) as compared with hybrid capture for a single mutation (1%). C, Influence of multiple markers and sequencing depth on LLOD. Downsampling of HPV-seq data from full-length dual-strand hybrid capture demonstrates the dependence of the LLOD on the targeted length of HPV-16 genome (i.e., number of markers; right y -axis) and the sequencing depth ( x -axis). The probability of detecting the indicated number of HPV molecules (1, blue circles; 2, gray triangles; 5, yellow squares) is shown (left y -axis).

    Journal: Clinical Cancer Research

    Article Title: HPV Sequencing Facilitates Ultrasensitive Detection of HPV Circulating Tumor DNA

    doi: 10.1158/1078-0432.CCR-19-2384

    Figure Lengend Snippet: Analytic sensitivity of HPV-seq. A, HPV-seq was conducted on fragmented SiHa genomic DNA at the indicated dilution. Hybrid capture baits targeted the indicated HPV-16 sequences. The lower limit of detection (LLOD) of HPV-seq was dependent on the use of dual-strand hybrid capture and the length of HPV-16 genome targeted by the baits. B, HPV-seq with full-length dual-strand hybrid capture (blue) provided an improvement in analytic sensitivity and LLOD (0.003%) as compared with hybrid capture for a single mutation (1%). C, Influence of multiple markers and sequencing depth on LLOD. Downsampling of HPV-seq data from full-length dual-strand hybrid capture demonstrates the dependence of the LLOD on the targeted length of HPV-16 genome (i.e., number of markers; right y -axis) and the sequencing depth ( x -axis). The probability of detecting the indicated number of HPV molecules (1, blue circles; 2, gray triangles; 5, yellow squares) is shown (left y -axis).

    Article Snippet: The SiHa cervix cancer cell line, which harbors 1 to 2 integrated copies of HPV-16 , was obtained from ATCC (catalog no. HTB-35, RRID:CVCL_0032).

    Techniques: Mutagenesis, Sequencing